标题：AKTIVITAS STIMULASI KOMPONEN BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscoe) PADA SEL LIMFOSIT B MANUSIA SECARA IN VITRO [Effects of Bioactive Compounds of Ginger (Zingiber officinale Roscoe) Root on B Cell Lymphocyte Function using In Vitro System] Tejasari 1), Fransiska R Zakaria 2) , dan Dondin Sajuthi 3) 1) Staf Pengajar Fakultas Teknologi Pertanian, Universitas Jember 2) Staf Pengajar Jurusan Teknologi Pangan dan Gizi, Fateta-IPB 3) Peneliti Pada Pusat Studi Satwa Primata IPB ABSTRACT The effect of two fractions of oleoresin compounds from ginger root on lymphocyte function were studied by observing B cell proliferations, which were measured by the incorporation of 3H-thymidine during cell incubation. The doses of oleoresin compounds and its two fractions tested were 50,100, 150, and 200 mg per ml. Lymphocyte from human peripheral blood were isolated using ficoll density gradient technique, and cultured in the presence of the compounds in RPMI-1640 medium for 4 days, with or without the addition of 3 mM paraquat as the oxidizing agent. The results showed that the effects of oleoresin and its two fractions depended on its doses. Oleoresin and its two fractions increased B cell proliferation with the highest activity of 456 percent at low dose of 50 mg per ml. These result showed a positive effect of oleoresin compound and its two fractions on B cell functions at low doses. It indicated that bioactive compounds of ginger root at low concentration have positive effects on humoral immune response and this supports the traditional belief that ginger increases body resistance to common cold. Key words : bioactive compound, proliferation, humoral immune responds, stress oxidative, in vitro, and lymphocyte
出版社：Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI
摘要：AKTIVITAS STIMULASI KOMPONEN BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscoe) PADA SEL LIMFOSIT B MANUSIA SECARA IN VITRO [Effects of Bioactive Compounds of Ginger (Zingiber officinale Roscoe) Root on B Cell Lymphocyte Function using In Vitro System] Tejasari 1), Fransiska R Zakaria 2) , dan Dondin Sajuthi 3) 1) Staf Pengajar Fakultas Teknologi Pertanian, Universitas Jember 2) Staf Pengajar Jurusan Teknologi Pangan dan Gizi, Fateta-IPB 3) Peneliti Pada Pusat Studi Satwa Primata IPB ABSTRACT The effect of two fractions of oleoresin compounds from ginger root on lymphocyte function were studied by observing B cell proliferations, which were measured by the incorporation of 3H-thymidine during cell incubation. The doses of oleoresin compounds and its two fractions tested were 50,100, 150, and 200 mg per ml. Lymphocyte from human peripheral blood were isolated using ficoll density gradient technique, and cultured in the presence of the compounds in RPMI-1640 medium for 4 days, with or without the addition of 3 mM paraquat as the oxidizing agent. The results showed that the effects of oleoresin and its two fractions depended on its doses. Oleoresin and its two fractions increased B cell proliferation with the highest activity of 456 percent at low dose of 50 mg per ml. These result showed a positive effect of oleoresin compound and its two fractions on B cell functions at low doses. It indicated that bioactive compounds of ginger root at low concentration have positive effects on humoral immune response and this supports the traditional belief that ginger increases body resistance to common cold. Key words : bioactive compound, proliferation, humoral immune responds, stress oxidative, in vitro, and lymphocyte
关键词：AKTIVITAS STIMULASI KOMPONEN BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscoe) PADA SEL LIMFOSIT B MANUSIA SECARA IN VITRO [Effects of Bioactive Compounds of Ginger (Zingiber officinale Roscoe) Root on B Cell Lymphocyte Function using In Vitro System] Tejasari 1); Fransiska R Zakaria 2) ; dan Dondin Sajuthi 3) 1) Staf Pengajar Fakultas Teknologi Pertanian; Universitas Jember 2) Staf Pengajar Jurusan Teknologi Pangan dan Gizi; Fateta-IPB 3) Peneliti Pada Pusat Studi Satwa Primata IPB ABSTRACT The effect of two fractions of oleoresin compounds from ginger root on lymphocyte function were studied by observing B cell proliferations; which were measured by the incorporation of 3H-thymidine during cell incubation. The doses of oleoresin compounds and its two fractions tested were 50;100; 150; and 200 mg per ml. Lymphocyte from human peripheral blood were isolated using ficoll density gradient technique; and cultured in the presence of the compounds in RPMI-1640 medium for 4 days; with or without the addition of 3 mM paraquat as the oxidizing agent. The results showed that the effects of oleoresin and its two fractions depended on its doses. Oleoresin and its two fractions increased B cell proliferation with the highest activity of 456 percent at low dose of 50 mg per ml. These result showed a positive effect of oleoresin compound and its two fractions on B cell functions at low doses. It indicated that bioactive compounds of ginger root at low concentration have positive effects on humoral immune response and this supports the traditional belief that ginger increases body resistance to common cold. Key words : bioactive compound; proliferation; humoral immune responds; stress oxidative; in vitro; and lymphocyte
