标题:AKTIVITAS ANTIBAKTERI EKSTRAK TEMPE TERHADAP BAKTERI Bacillus subtilis DAN Staphylococcus aureus [Antibacterial Activity of Tempe Extracts on Bacillus subtilis and Staphylococcus aureus]
出版社:Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI
摘要:AKTIVITAS ANTIBAKTERI EKSTRAK TEMPE TERHADAP BAKTERI Bacillus subtilis DAN Staphylococcus aureus [ Antibacterial Activity of Tempe Extracts on Bacillus subtilis and Staphylococcus aureus ] D. Elysa Putri Mambang 1) *, Rosidah 1 ) dan Dwi Suryanto 2 ) 1) Program Studi Magister Farmasi Fakultas Farmasi, Universitas Sumatera Utara, Medan 2 ) Departemen Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan Diterima 13 Agustus 2013 / Disetujui 05 Februari 2014 ABSTRACT Tempe is a soybean fermented product by Rhizopus oligosporus which contain s high amounts of bioactive comp o unds including antibacterial compounds. The aim of this research i s to observe the antibacterial activity of tempe extract against Baci l lus subtilis and Staphylococcus aureus . The results of this research broadens the current knowledge of tempe as functional food. Samples of c ommercial tempe were obtained from a traditional market of Pringgan Medan, Sumatera Utara. Tempe samples were then extracted using three solvent s with different polarity: ethanol, n-hexane and ethyl acetate , each at three levels of concentration i.e. 300 mg/mL (30%), 400 mg/mL (40%) and 500 mg/mL (50%). The a ntibacterial activity test of tempe extracts was carried out using disc difussion method on Mueller Hinton Agar (MHA), and incubation was conducted at 36-37°C for 24 hr s . The obtained data were tested statistically with Analysis of Variant (ANOVA) p>0.05. The ethanol and ethyl acetate extract s of tempe inhibited the growth of B. subtilis and S. aureus . Meanwhile, the ethyl acetate extract of tempe at concentration of 500 mg/m L had the highest inhibition against S. aureus as shown by inhibition diameters of 15.50±0.44 mm (p>0.05), and B. subtilis at 14.13±0.21 mm (p>0.05). No inhibition observed with the n-hexane extracts of tempe again st the above bacteria.