期刊名称：Journal of Natural and Environmental Sciences
印刷版ISSN：1309-7474
出版年度：2011
卷号：2
期号：2
页码：25-31
语种：English
出版社：Journal of Natural and Environmental Sciences
摘要：The development of antibiotic resistance in the environment can contribute to decline in the effectiveness of antibiotic treatment of diseases. Currently antibiotic resistance can be found in a wide range of environments and the occurrence of antibiotic resistance has been linked to human activities such as urbanization and agriculture. The purpose of this study was to determine the effects of land use (agricultural and urban), environmental media (streambed sediment and water), and season (spring/summer and fall/winter) on the occurrence of tetracycline resistant bacteria and 15 tetracycline resistance genes in a rapidly urbanizing perennial stream in Texas. This study utilized both culture based and molecular methods to evaluate the occurrence of resistant bacteria and resistance genes at five sampling locations with various landuse along the stream. Samples were collected five times during the spring/summer season (between March and August) and five times during the fall/winter season (between September and February).The results from this study showed that tetracycline resistant bacteria were capable of growth at both low (0.03 mM) and high (0.06 mM) tetracycline levels in all environmental samples, and tet(W) was detected in all samples. Further analysis showed that the concentration of tetracycline resistant bacteria in streambed sediments was higher than in stream water. However, the occurrence of tetracycline resistance genes was not significantly different. The data also showed that season had no significant effect on the occurrence of tetracycline resistant bacteria or resistance genes. Finally, the results indicated that landuse had no significant effect on the concentration of tetracycline resistant bacteria but did significantly increase the occurrence of resistance genes in this watershed. This study illustrated that rapidly urbanizing watersheds may contribute to the occurrence and prevalence of tetracycline resistance in the environment. This finding is supported by the presence of tetracycline resistant bacteria and tetracycline resistance genes in all sediment and surface water samples. Wastewater treatment facilities did not appear to contribute to an increase in resistant bacteria concentration; however they did contribute to an increase in resistance genes. This indicates that these facilities might contribute to the diversity of resistance genes in a watershed.
关键词：Environmental Engineering;Emerging Contaminants, Antibiotic Resistance Genes, Water Quality;Surface Water Quality
