摘要：A molecular-based study was conducted to estimate the richness of the V ibrio on tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimp s were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopan c reas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR) , it was found that two groups of V ibrio. To investige the efectiveness of rep-PCR in estimating the richness of V ibrio on tiger shrimps, three isolates (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus , respectively. The result proved that two assosiated of V ibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore the present study highlight s the effectiveness of rep-PCR in rapid grouping and estimating the richness of V ibrio on tiger shrimp.
关键词：Rep-PCR;Vibriosis;Causative Agent;Penaeus monodon Fab
