期刊名称：Journal of Plant Molecular Biology and Biotechnology
印刷版ISSN：2146-0302
出版年度：2012
卷号：3
期号：2
页码：1-10
语种：English
出版社：Journal of Plant Molecular Biology and Biotechnology
摘要：A current, real-time fingerprinting application being used commercially in Canada is the fingerprinting of two different wheat varieties within a midge, Sitodiplosis mosellana (Ge/hin), tolerant blend. A proportion of susceptible (refuge, 10%) plants are interspersed or in close proximity to resistant (Sm-1 resistance, 90%) plants which prevents or delays the development of insect resistance. This mixture ratio must be monitored over successive wheat generations since high rates of variety (genetic) drift different from the 90:10 ratio can occur. A varietal blend is a new concept not previously applied for in certified seed sales and the Cdn Seed Growers Association (CSGA) and Canadian Food & Inspection Agency (CFIA) need information on the stability of these blends. These blends significantly increase wheat yields especially in high midge pressure environments. We describe a cost-effective, SNP-scoring platform for blend monitoring in detail where thousands of wheat samples/ seeds are processed simultaneously in a short time. Single seed analysis and single nucleotide polymorphism (SNP) markers are used in the Rapid ID Technology (RIDT) platform. The RIDT platform genotypes by the Invader assay in microwells and up to 1,000 single seeds can be fingerprinted individually and simultaneously under 5 hours, from “seed to base call". Over 50,000 single seeds were genotyped for 4 different genetic blends/ 8 locations and there was no significant departure from the 90: 10 ratio over 3-4 successive generations.
