期刊名称：Karbala International Journal of Modern Science
印刷版ISSN：2405-609X
电子版ISSN：2405-609X
出版年度：2016
卷号：2
期号：3
页码：156-168
DOI：10.1016/j.kijoms.2016.03.006
语种：English
出版社：Elsevier
摘要：Abstract Pest management tools should rely to proper identification of arthropod species, which are usually classified relying on morphological keys. However, the shortcomings and limitations of the conventional taxonomical identification methods highlighted need for new and simple methods of pest identification. In this research, DNA barcoding was used to identify 15 insect pests attacking horticultural crops in South India. Accurate phylogenetic information and evolutionary divergence data were supported and evidenced by various parameters, including the rates of substitution, nucleotide composition, genetic divergence, test of selection and saturation analysis. The null hypothesis of neutral selection was rejected in favour of the alternate hypothesis of purifying selection. NJ and ML trees were calculated and the individuals of the same species clustered together based on the CO1 sequence similarity, regardless of their collection site and geographic location. Overall, this study adds basic knowledge to molecular ecology of important insect pests attacking horticultural crops in South India.
关键词：Insect pest; DNA barcoding; Molecular ecology; Nucleotide divergence; Phylogenetics;
