摘要：Abstract The enhancement in immobilization process using microbial with organic matrix in biological process was an extensive research. The novel organic matric was found to be used in immobilized process. The application of bamboo as microbial matrix in immobilization process was presented. The physical characteristic of bamboo was studied. The nature physical characteristic such as high porosity promotes bamboo as an alternative microbial matrix. The use of Bambusa heterostachya or locally called as ‘buluh galah’ as organic matrix is a dynamic microbial environment. The existent of microbial cells inside bamboo by immobilization process are optimally structured the use of matrix. The microorganism, Lactobacillus delbrueckii was selected as microbial used in immobilized of bambusa matrix. The effect study was interaction time with constant pH of 7 and temperature, 37˚C. It was performed to determine the bacterial attach in bambusa matrix during immobilized process for every 2hours using scanning electron microscopy (SEM). The results show that the interaction time of immobilized process on Bambusa heterostachya as Lactobacillus matrix was interactive at 24hours of inoculation process. Before the immobilization process, scanning electron microscope (SEM) showed that the pore tissue inside bamboo was empty with bacterial. However, after 24hours of inoculation process, analysis showed that the pore size of bamboo was full with Lactobacillus. Therefore, this study proved that bamboo is a good matrix for microbial growth. The immobilized process using Bambusa heterostachya as Lactobacillus matrix can be further use in industrial application especially in the field of wastewater treatment.
