期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2017
卷号：6
期号：1
页码：87-95
DOI：10.1016/j.bjbas.2016.11.010
语种：English
出版社：Elsevier
摘要：Highlights • Thermomyces lanuginosus strains were used in the present study was isolated from Warangal, Telangana, India and were confirmed with 28 S rDNA genome sequences were genetically different from each other. • The maximum lipase was produced by GSLMBKU-10 and GSLMBKU-13 in YES medium with triacetene. • The stimulatory production was recorded at 6.0, 6.5 and 7.0 by GSLMBKU-10, GSLMBKU-13 and SLMBKU-14 respectively. • Furthermore, temperature of 45°C was optimum for secretion of lipase by GSLMBKU-14 and GSLMBKU-10, while GSLMBKU-13 it was at 50°C. Abstract In the present investigation lipase production by three strains of thermophilic Thermomyces lanuginosus (GSLMBKU-10, GSLMBKU-13 and GSLMBKU-14) was carried out in submerged fermentation process. Olive oil and triacetin (0.1%) were added to the basal medium, which stimulated the lipase production. The maximum lipase was produced by GSLMBKU-10 and GSLMBKU-13 in yeast extract starch medium supplemented with triacetin (0.1%). The optimum pH was recorded at 6.0, 6.5 and 7.0 by GSLMBKU-10, GSLMBKU-13 and GSLMBKU-14 respectively. T. lanuginosus GSLMBKU-10 strains failed to produce lipase at pH 8.0. The optimum temperature for lipase production was observed at 45 °C by GSLMBKU-14 and GSLMBKU-10, while that for GSLMBKU-13 was at 50 °C. The marginal temperature ranged from 45 °C to 50 °C for both lipase production and vegetative growth by the three strains of T. lanuginosus under study. In conclusion, the GSLMBKU-13 strain was comparatively superior in the production of lipase than the other two strains under investigation.
关键词：Thermomyces lanuginosus;Oils;Culture medium;pH;Temperature;Lipase production
