标题：Cypermethrin-induced histopathological, ultrastructural and biochemical changes in liver of albino rats: The protective role of propolis and curcumin
期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2017
卷号：6
期号：2
页码：160-173
DOI：10.1016/j.bjbas.2017.03.002
语种：English
出版社：Elsevier
摘要：Highlights • The study tended to clarify the protective effect of PRO and CUR against toxicity of cypermethrin on some organs. • Evaluate the effects of treatment with PRO and CUR in the various control and tested groups. • Administration of PRO and CUR are beneficial to improve biochemical, histopathological and ultrastructural due to their antioxidants effects. • The protective effects of PRO are markedly effective than CUR against CYP. Abstract Cypermethrin (CYP), an insecticide belongs to a synthetic pyrethroid, is used for agriculture and household applications. The present study aimed to examine the toxic effects of CYP on rat liver and to clarify the hepatoprotective effects of propolis (PRO) and curcumin (CUR) against CYP. This study was assessed in male albino rats, each weighting (120–150g). The rats were equally divided into six groups as follow; the 1st control group, 2nd PRO group (100mg/kg/day) and 3rd CUR group (100mg/kg/day). While 4th, 5th and 6th groups were orally treated with CYP (30mg/kg/day), CYP plus PRO and CYP plus CUR, respectively for 28days. The present study revealed that CYP-induced significant increase in hepatic markers enzymes (ALT, AST and ALP) and elevation in MDA concomitant with significant decrease of SOD and GPx levels. Histological and histochemical results revealed extensive vacuolar degeneration of hepatocytes, fatty change, blood vessel congestion and fibrosis in the liver of CYP- treated group and depletion of glycogen, protein and DNA. Moreover, ultrastructural observations showed vacuolation, damage of mitochondria and nuclear changes. On the other hand, treatment with PRO and CUR led to an obvious improvement of the injured liver tissues and ameliorating the damaging effects of CYP. In conclusion, PRO is markedly effective than CUR in protecting rats against CYP-induced histopathological, ultrastructural and biochemical changes. This protection may be due to its antioxidant properties and scavenging abilities against active free radicals.
关键词：Cypermethrin;Oxidative stress;Histopathology;Ultrastructure;Propolis and curcumin
