期刊名称:Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN:2314-8535
电子版ISSN:2314-8543
出版年度:2017
卷号:6
期号:2
页码:209-217
DOI:10.1016/j.bjbas.2017.04.002
语种:English
出版社:Elsevier
摘要:Abstract The present study aimed to isolate actinomycetes from soil samples collected from Western Ghats regions, Tamil Nadu, India. Totally 400 actinomycetes isolates were isolated, of which 161 (40.2%) showed antimicrobial activity in preliminary screening. Secondary screening yielded 27 active isolates; twelve of them showed good antimicrobial and larvicidal activities. Five isolates namely S12-4, S12-17, S12-8, DPS-14 and FMS-20 presented 98%, 93%, 87%, 82%, and 80% larvicidal activity against Cx. quinquefasciatus; very low larvicidal activity was observed against Ae. aegypti. S12-4 showed 100% larval mortality against An. stephensi. RAPD results showed highest similarity between DPS-122 and KFS-24 (0.89246). The polymorphic index observed in the primers ranged from 0.286 (OPA-09) to 0.07 (OPC-18). The results suggest that Western Ghats regions may be a good natural source for isolation of actinobacteria and thereby for discovering novel molecules with antimicrobial and mosquito larvicidal properties.
关键词:Antimicrobial activity;Larvicidal bioassay;RAPD;Culex quinquefasciatus;Anopheles stephensi;Aedes aegypti