期刊名称：Karbala International Journal of Modern Science
印刷版ISSN：2405-609X
电子版ISSN：2405-609X
出版年度：2017
卷号：3
期号：2
页码：69-74
DOI：10.1016/j.kijoms.2017.02.003
语种：English
出版社：Elsevier
摘要：Abstract Background Staphylococcal Food Poisoning (SFP) is an intoxication caused by consumption of improperly prepared or stored foods containing adequate amounts of one (or more) preformed enterotoxin. Previous studies demonstrated that employees who working in the food industry are the main source for spreading foodborne diseases. Aims To study the prevalence of the staphylococcal enterotoxin genes among the isolates from the food handlers in Holy Kerbala city. Materials and methods Nasal swabs were collected from 332 food handlers. Standard microbiological methods were used to isolate Staphylococcus aureus including, culturing on selective media (mannitol salt agar), coagulase test and API-staph. Multiplex PCR (Polymerase Chain Reaction) using specific primers was used for the detection of staphylococcal enterotoxin genes. Result and discussion 100 food handlers out of 332 (30.1%) were found to carry S. aureus, 38 (38%) isolates were found positive in Multiplex PCR for one or more enterotoxin: 16 (16%) were positive for sea, 18 (18%) were positive for seb, 8 (8%) were positive for sec, 6 (6%) were positive for sed and 8 (8%) were positive for see. Conclusion The prevalence of nasal carriage of S. aureus is high among food handlers in Holy Kerbala city, as well as the percentage of enterotoxin genes among the S. aureus isolates. Therefore, strict measures are necessary to prevent contamination of food with S. aureus isolates during food handling.
