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  • 标题:Up-Streaming Process for Glucose Oxidase by Thermophilic Penicillium sp. in Shake Flask
  • 本地全文:下载
  • 作者:Muhammad Mohsin JAVED ; Aroosh SHABIR ; Sana ZAHOOR
  • 期刊名称:Walailak Journal of Science and Technology (WJST)
  • 印刷版ISSN:2228-835X
  • 出版年度:2012
  • 卷号:9
  • 期号:2
  • 页码:127-133
  • DOI:10.2004/wjst.v9i2.216
  • 语种:English
  • 出版社:Institute of Research and Development, Walailak University.
  • 摘要:The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp . in 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. Effect of submerged fermentation in shaking and static conditions, different carbon sources and incubation period on the production of extra-cellular glucose oxidase was studied. Maximum yield (0.325 U/mL) of extra-cellular glucose oxidase was obtained after 72 hrs of incubation at 45 o C using submerged fermentation technique in shaking conditions utilizing sucrose as carbon source.
  • 关键词:Glucose oxidase, thermophilic, Penicillium, submerged fermentation
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