期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2012
卷号：9
期号：4
页码：347-359
DOI：10.2004/wjst.v9i4.454
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Effects of media compositions on biomass and lipid accumulation of the isolate Chlorella sp. TISTR 8990 were investigated under a Plackett-Burman experimental design with mixotrophic cultivation conditions. Under this experimental design there were 15 different runs with ten factors-yeast extract, KH 2 PO 4 , MgSO 4 , FeSO 4 , MnCl 2 , CuSO 4 , Na 2 MoO 4 , H 3 BO 3 , ZnSO 4 and pH. Cultures were grown mixotrophically under 16 h light and 8 h dark regime at 30 ºC for a period of 7 days. During the light regime, the light intensity at the surface of the vessels and agitation speed were set to 67.5 µmol photons m -2 s -1 and 150 rpm, respectively. Initial cell concentration was set to an absorbance (A 540 ) of 0.5. For high biomass production (2.2 g/L, run no. 6), the most effective and significant factors were yeast extract, KH 2 PO 4 , FeSO 4 and ZnSO 4 at concentrations 0.3 g/L, 0.3 g/L, 3 mg/L and 0.3 mg/L, respectively. Whereas for high lipid accumulation (19.59 %DCW, run no. 2), these were KH 2 PO 4 , pH and yeast extract, at a level of 1.7 g/L, 6.0 and 0.1 g/L, respectively. No significant factors were obtained for higher lipid content. The best treatment for biomass and lipid content was run no. 6, whose medium formula consisted of 0.3 g/L yeast extract, 1.7 g/L KH 2 PO 4 , 1.7 g/L MgSO 4 , 1 mg/L FeSO 4 , 0.9 mg/L MnCl 2 , and pH 7.0, together with fixed concentrations of glucose, NaHCO 3 and KNO 3 at 5 g/L, 0.05 g/L and 0.5 g/L, respectively.
关键词：Biomass, Chlorella sp., mixotroph, Plackett-Burman design, single cell oils
