期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2014
卷号：12
期号：4
DOI：10.14456/867
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：Barringtonia racemosa and Barringtonia spicata are moderate sized trees that can be found in Southeast Asia. They are medicinal plants that were used in traditional practice to treat itch, high blood pressure and as a depurative from their leaves. Due to its healing properties of the leaves, it is relevant to determine the antioxidant properties and total phenolic contents from different developmental stages of the leaves. It was assumed that the young leaf have higher activity than mature ones. The total phenolic contents (TPC) of the plant extract was determined besides the free radical scavenging ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and also the capacity of extracts to inhibit lipid peroxidation by ferric thiocyanate (FTC) and thiobarbituric (TBA) assay. The highest TPC was observed in the methanolic extracts of stage one leaf of B. racemosa (0.34 mg GAE / g DW) and B. spicata (0.19 mg GAE / g DW). The highest scavenging activity was shown from methanolic extract from stage three leaf of B. racemosa with IC50 at 33.33 µg/ml and in B. spicata it was shown in stage four at 42.33 µg/ml as compared to the chloroform extraction. All extracts were actively inhibiting lipid peroxidation with more than 88% of inhibition. The healing properties of Barringtonia could be from their high antioxidant activities. More detailed study will be required to identify which of the active compounds are present in the leaves that were act as antioxidant compounds.
关键词：Plant Science;Barringtonia, free radical scavengers, lipid peroxidation, total phenolic contents
