摘要：In this study, the effects of leukotriene receptor antagonist on mRNA expression of MUC2 and MUC5B in human nasal epithelial cells were investigated. Human nasal epithelial cells were randomly assigned into 4 groups: control group, IL-1β group, montelukast + IL-1β group and montelukast group. After 24 h of treatment, the mRNA expressions of MUC2 and MUC5B were determined by fluorescent quantitation PCR. The mRNA expression of MUC2 in the control group, IL-1β group, montelukast + IL-1β group and montelukast group was 2.93±1.57×106copies/μg, 6.63±1.73×106 copies/μg, 3.48±1.41)×106 copies/μg and 1.63±0.47×106 copies/μg, respectively(P<0.01). The MUC2 level in montelukast + IL-1β group was lower than in IL-1β group (P<0.01), but there was no marked difference between montelukast group and control group. The mRNA expression of MUC5B in control group, IL-1β group, montelukast + IL-1β group and montelukast group was 8.21±3.54×105 copies/μg, 3.40±2.79×107 copies/μg, 1.75±0.69×106copies/μg and 5.15±2.16×105 copies/μg, respectively, showing significant difference among these groups (P<0.01). In addition, the MUC5B level in montelukast + IL-1β group was lower than in IL-1β group (P<0.05), but there was no marked difference between the montelukast and the control group. Montelukast, a leukotriene receptor antagonist, can decrease the mRNA expressions of MUC2 and MUC5B in IL-1β treated nasal epithelial cells. Our results reveal montelukast may inhibit the inflammatory cytokine induced expression of mucins in nasal epithelial cells.
关键词：Montelukast; leukotriene; MUC2; MUC5B; human nasal epithelial cells
