摘要：Considering the heavy losses caused by pear ring rot (Botryosphaeria berengeriana) disease, the potential biocontrol agent againstB. berengerianawas isolated and characterized in this work. Bacteria, isolated from pear orchard rhizosphere soil, were screened for the biological control of pear ring rot caused byB. berengeriana.Among them, strain L-1 showed noticeable inhibitory activities againstB. berengerianaand six other commonly occurring postharvest pathogens. Molecular methods indicated strain L-1 wasBacillus amyloliquefaciens.The potential of strain L-1 as an effective biocontrol agent was further estimated. Results showed strain L-1 could successfully colonize in pear wounds, its colonies reached 142.35 folds on 4 days post inoculation, and maintained at a high level during storage. In addition, strain L-1 caused abnormal hyphae growth ofB. berengeriana, and its inhibitory percentage against pear ring rot reached 76.55%in vivoon 11 days post inoculation. Strain L-1 significantly induced the peroxidase (POD) and catalase (CAT) activities and delayed the accumulation of malondialdehyde (MDA) in pears. What's more, strain L-1 did not impair the fruit quality. All these results suggest thatB. berengerianaL-1 is a promising agent for the biocontrol of pear ring rot.
关键词：pear ring rotBacillus amyloliquefaciensbiological controlfruit quality parameters
