期刊名称：Journal of Radiation Research and Applied Sciences
印刷版ISSN：1687-8507
出版年度：2015
卷号：8
期号：2
页码：234-242
DOI：10.1016/j.jrras.2015.01.010
出版社：Elsevier B.V.
摘要：AbstractPurpose The present study was conducted to characterize the possible therapeutic effects of glucagon-like peptide (GLP)-1 and GLP-2 against oxidative damage in the ileum and colon of irradiated rats. Methods and materials Sprague-Dawley rats of both sexes received either a single dose of GLP-1 (0.1 nmol/kg, intraperitoneally, ip; n = 6) 10 min before abdominal irradiation (IR) or two consecutive doses of GLP-2 (7 nmol/kg, ip; n = 6) at 30 and 10 min before IR, while another group was administered vehicle (n = 6) 10 min before IR. Control rats (n = 6) received vehicle treatment without IR. On the fourth day of IR, samples from ileum and colon were removed for histological analysis, for the determination of myeloperoxidase (MPO) activity, malondialdehyde (MDA) and glutathione (GSH) levels, as well as \{DNA\} fragmentation ratio, an index of apoptosis. Results IR-induced oxidative injury in the colonic tissue of vehicle-treated rats, evidenced by elevated \{MDA\} levels and \{MPO\} activity, as well as depleted colonic \{GSH\} levels, was reversed by GLP-2, while GLP-1 reduced IR-induced elevations in colonic \{MDA\} levels. IR-induced injury with elevated ileal \{MDA\} levels was reduced by GLP-1, while replenishment in \{GSH\} was observed in GLP-2-treated rats. Conclusion Current findings suggest that GLP-1 and GLP-2 appear to have protective roles in the irradiation-induced oxidative damage of the gut by inhibiting neutrophil infiltration and subsequent activation of inflammatory mediators that induce lipid peroxidation.
Loading...
