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  • 标题:A simple and fast method for extraction and quantification of cryptophyte phycoerythrin
  • 本地全文:下载
  • 作者:Christina Thoisen ; Benni Winding Hansen ; Søren Laurentius Nielsen
  • 期刊名称:MethodsX
  • 印刷版ISSN:2215-0161
  • 电子版ISSN:2215-0161
  • 出版年度:2017
  • 卷号:4
  • 页码:209-213
  • DOI:10.1016/j.mex.2017.06.002
  • 语种:English
  • 出版社:Elsevier
  • 摘要:Graphical abstractDisplay OmittedAbstractThe microalgal pigment phycoerythrin (PE) is of commercial interest as natural colorant in food and cosmetics, as well as fluoroprobes for laboratory analysis. Several methods for extraction and quantification of PE are available but they comprise typically various extraction buffers, repetitive freeze-thaw cycles and liquid nitrogen, making extraction procedures more complicated. A simple method for extraction of PE from cryptophytes is described using standard laboratory materials and equipment. The cryptophyte cells on the filters were disrupted at −80°C and added phosphate buffer for extraction at 4°C followed by absorbance measurement. The cryptophyteRhodomonas salinawas used as a model organism.•Simple method for extraction and quantification of phycoerythrin from cryptophytes.•Minimal usage of equipment and chemicals, and low labor costs.•Applicable for industrial and biological purposes.
  • 关键词:Labor cost;Microalgae;Rhodomonas salina;Pigment;Phycobiliprotein;Processing time
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