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  • 标题:Algal density assessed by spectrophotometry: A calibration curve for the unicellular algae Pseudokirchneriella subcapitata
  • 本地全文:下载
  • 作者:Lúcia Helena Ribeiro Rodrigues ; Alexandre Arenzon ; Maria Teresa Raya-Rodriguez
  • 期刊名称:Journal of Environmental Chemistry and Ecotoxicology
  • 电子版ISSN:2141-226X
  • 出版年度:2011
  • 卷号:3
  • 期号:8
  • 页码:225-228
  • 语种:English
  • 出版社:Academic Journals
  • 摘要:The unicellular algaePseudokirchneriella subcapitata(Korschikov) Hindák has been frequently used forecotoxicological tests.In this paper, a calibration curve is proposed correlating absorbance values (684 nm) and cell density for routine ecotoxicological experiments. Density (cells/mL) could be estimated as follow: Cell Density = e{[ln (absorbance_684) +16.439]/1.0219}(n=130; r2=0.9998). Residual distribution revealed that the equation could be applied for algal densities under 5,000,000 cells/mL and/or absorbance values as high as 0.5.
  • 关键词:Pseudokirchneriella subcapitata ; spectrophotometry; ecotoxicological tests.
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