期刊名称：International Journal of Environment and Bioenergy
电子版ISSN：2165-8951
出版年度：2014
卷号：9
期号：3
页码：243-251
语种：English
出版社：Modern Scientific Press
摘要：Red macroalgae Gelidium sp. has been used for production of agar and then bioethanol since it has high carbohydrate content. This study focused on hydrolysis of agar of Gelidium sp. by agarases enzyme that produced from marine bacteria that was collected from different genera of bacteria which found in seawater, marine sediments, and another microorganisms. The optimal enzyme stability concentration for Gelidium sp. hydrolysis was achieved in terms of total reducing sugar at 664 ppm, 10% concentration. Results of protein molecule measured by sodium dodecyl sulphate polyacrylamide gel (SDS PAGE) method was 37 kDa, 41 kDa and 52 kDa respectively. Hydrolysis of Gelidium sp. produce 1.04% bioethanol and species of bacteria identified using moleculer 16 sDNA was Pseudomonas stutzeri. Marine bacteria showed potential to hydrolyse red macroalgae for producing bioethanol.
