期刊名称：International Journal of Environment and Bioenergy
电子版ISSN：2165-8951
出版年度：2012
卷号：3
期号：3
页码：145-153
语种：English
出版社：Modern Scientific Press
摘要：Peat soil composes of organic compounds derived from plant debris with organic carbon content higher than 25%. The organic fraction of peat soil generally contains lignin, cellulose, hemicellulose, proteins, waxes, tannins, resins, and suberin. In this study, the cellulolytic bacteria were isolated from peat soils from Ogan Komering Ilir, South Sumatera, Indonesia, tested for their cellulase activities, and the potential isolates were identified into genus or species level. The degradation ability of cellulose was screened using 1% CMC (carboxy methyl cellulose) as a substrate and the cellulase activities of crude enzyme were determinated using DNS (3,5-dinitro salicyclic acid) method. As results, we selected four bacterial isolates (S3B40, S3B32, S3B37, and AB16) that have high cellulase activities. Among 4 isolates, Paenibacillus elgii S3B40 has the highest cellulase activity in all tested. Optimum activity of isolate S3B40 at pH 8 and temperature 60 ℃ was 14.5 U/mL, S3B32 at pH 8 and temperature 80 ℃ was 0.506 U/mL, AB16 at pH 5 and temperature 50 ℃ was 0.361 U/mL, and S3B37 at pH 5 and temperature 30 ℃ was 1.167 U/mL, respectively.
