摘要:Phenolic compounds are hazardous pollutants that are toxic relatively at low concentrations. Accumulation of phenol creates toxicity both for flora and fauna. Because of its toxicity, there is a need to decontaminate the phenol-laden soils. Hence, bioremediation is a very useful alternative to conventional clean-up methods. The aim of this work was to study the effect of two variables – temperature (300C, 320C, 330C, 340C) and carbon (0.5, 1, 2, 3, 4 gm/L) to identify the significant effects and interactions in the batch studies. It was found that the degrading potential of Pseudomonas aeruginosa (NCIM 2074) was strongly affected by the variations in carbon and temperature. Optimum conditions of the variables for the growth of P. aeruginosa (NCIM 2074) and for maximum biodegradation of phenol are temperature (320C) and carbon (0.5gm/L). These results are useful to understand the physiological and biochemical properties of P. aeruginosa (NCIM 2074) before its optimum use in environmental application and these data will assist in choosing the right phenol degrader for a changeable environment.