标题：Pulmonary Pseudomonas aeruginosa infection induces autophagy and proteasome proteolytic pathways in skeletal muscles: effects of a pressurized whey protein-based diet in mice
摘要：ABSTRACTBackground: PulmonaryPseudomonas aeruginosainfection in cystic fibrosis patients is associated with skeletal muscle atrophy. In this study, we investigated the effects ofP. aeurginosainfection and a whey protein-rich diet on skeletal muscle proteolytic pathways.Design: An agar bead model of pulmonaryP. aeurginosainfection was established in adult C57/Bl6 mice. Protein ubiquitinaiton, lipidation of LC3B protein and expressions of autophagy-related genes and ubiquitin E3 ligases were quantified using immunoblotting and qPCR. The effects of pressure-treated whey protein diet on muscle proteolysis were also evaluated.Results: PulmonaryP. aeurginosainfection reduced diaphragm, tibialis anterior, and soleus muscle weights and increased protein ubiquitination, LC3B protein lipidation, and the expressions ofLc3b,Gabarapl1,Bnip3,Parkin, Atrogin-1, andMuRF1genes in each muscle. These changes were greater in the tibialis as compared to soleus and diaphragm. Proteolysis indicators increased within one day of infection but were not evident after seven days of infection. A pressurized whey diet attenuated LC3B protein lipidation, expressions of autophagy-related genes (BNIP3), pro-inflammatory cytokines, and protein ubiquitination.Conclusions: We conclude that pulmonaryP. aeruginosainfection activates the autophagy, and the proteasome pathways in skeletal muscles and that a pressurized whey protein diet attenuates muscle proteolysis in this model.
