标题：Evaluation of the phytochemical composition and protective activities of methanolic extracts of Centaurea borysthenica and Centaurea daghestanica (Lipsky) Wagenitz on cardiomyocytes treated with doxorubicin
摘要：ABSTRACTCentaureaL. is a genus of the familyAsteraceaethat comprises over 600 taxa. Representatives of theCentaureagenus were used as natural medications for many diseases. Methanolic-aqueous extracts from aerial parts of twoCentaureaspecies:C. borysthenicaGruner andC. daghestanica(Lipsky) Wagenitz were studied for their polyphenolic composition and potential protective effect on cardiomyocytes treated with doxorubicin. Effectiveness of doxorubicin in cancer therapy is limited by a dose-dependent cardiotoxicity. Oxidative stress is a widely recognized mechanism of this phenomenon. One of the most important strategies has been an application of drug together with antioxidant agents. A cardioprotective effect of selected extracts ofCentaureaspecies was suspected in this study. Cell viability, oxidative stress, and mitochondrial membrane potential analyses showed protective activity of the methanolic extract ofC. borysthenicaandC.daghestanicaon rat cardiomyocytes treated with doxorubicin. AlthoughC. borysthenicais more effective as a cardiomyocyte protective agent, in higher concentrations it weakened the drug activity.C. daghestanicaextract did not change the doxorubicin efficacy in the evaluated experiment. Interestingly, both tested extracts were cytotoxic for myeloma cells. The detected antioxidant activity of the studied extracts can be used in the prevention of doxorubicin-induced cardiotoxicity.
