期刊名称:African Journal of Environmental Science and Technology
电子版ISSN:1996-0786
出版年度:2012
卷号:6
期号:2
页码:104-114
DOI:10.5897/AJEST11.067
语种:English
出版社:Academic Journals
摘要:he use of pesticides is very critical in protecting the farmers’ investment in seeds, fertilizer and labour since they provide a sure cover from damage by pests. The use of pesticides is therefore inevitable and the environmental pollution due to pesticides and their residues will continue to be a challenge. In this study, bacterial strains capable of degrading methomyl (S-methyl-N-[(methylcarbamoyl) oxy]-thioacetimidate) and carbofuran (2, 3-dihydro-2, 2-dimethyl-7-benzofuranyl methylcarbamate) were isolated from soils sampled from horticultural farms with a history of pesticide usage. High pressure liquid chromatography was used to monitor biodegradation of both methomyl and carbofuran using reference standards and acetonitrile and water as mobile phases. Partial 16S rDNA sequence analysis indicated that the carbofuran-degrading strains were closely related to members of the genusPseudomonasandAlcaligeneswhile the methomyl degrading strains were closely related to members of the genusFlavobacteriumandAlcaligenes.The morphological and biochemical characteristics of the isolates also confirmed the phylogenetic signature. The study established that the activities of the esterase and phosphatase enzymes correlated well with biodegradative capability and recommends possible application of the isolates in thein vivobioremediation of pesticide contaminated soils.