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  • 标题:Characterization of methomyl and carbofuran degrading-bacteria from soils of horticultural farms in Rift Valley and Central Kenya
  • 本地全文:下载
  • 作者:Kevin Mbogo Omolo ; Gabriel Magoma ; Kamau Ngamau
  • 期刊名称:African Journal of Environmental Science and Technology
  • 电子版ISSN:1996-0786
  • 出版年度:2012
  • 卷号:6
  • 期号:2
  • 页码:104-114
  • DOI:10.5897/AJEST11.067
  • 语种:English
  • 出版社:Academic Journals
  • 摘要:he use of pesticides is very critical in protecting the farmers’ investment in seeds, fertilizer and labour since they provide a sure cover from damage by pests. The use of pesticides is therefore inevitable and the environmental pollution due to pesticides and their residues will continue to be a challenge. In this study, bacterial strains capable of degrading methomyl (S-methyl-N-[(methylcarbamoyl) oxy]-thioacetimidate) and carbofuran (2, 3-dihydro-2, 2-dimethyl-7-benzofuranyl methylcarbamate) were isolated from soils sampled from horticultural farms with a history of pesticide usage. High pressure liquid chromatography was used to monitor biodegradation of both methomyl and carbofuran using reference standards and acetonitrile and water as mobile phases. Partial 16S rDNA sequence analysis indicated that the carbofuran-degrading strains were closely related to members of the genusPseudomonasandAlcaligeneswhile the methomyl degrading strains were closely related to members of the genusFlavobacteriumandAlcaligenes.The morphological and biochemical characteristics of the isolates also confirmed the phylogenetic signature. The study established that the activities of the esterase and phosphatase enzymes correlated well with biodegradative capability and recommends possible application of the isolates in thein vivobioremediation of pesticide contaminated soils.
  • 关键词:Pesticides; carbofuran; methomyl; biodegradation; bacteria
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