摘要：The objective of this study was to isolate bacterial species capable of degrading Pyrasulfotole herbicide from the environment. A soil bacterium capable of degradation of herbicide was isolated from enrichment technique method. The soil samples were collected from agricultural fields of Sindh (Pir Jo Goth, Khairpur Mirus) and Punjab (Chak Waris Abad). The specific compound i.e. Pyrasul-fotole belongs to a chemical class of pyrazoles was first introduced in North American and Australian markets. In enrichment technique method the soil samples were treated with dose of Pyrasulfotole (6 times at interval of 10 days). During the whole process the flask was placed in the shaking incubator. The treatment was given to enrich the soil with herbicide degrading bacteria. These isolates were cultured on MSM (mineral salt media) plates and after growth further purified on nutrient agar plates. The identification after morphological characterization and biochemical test shows two types of bacterial species which have capability to degrade Pyrasulfotole. The bacterial species comprise Micrococcus arborescens and Brachybacterium sp. found to be responsible for degradation of Pyrasul-fotole.
