标题：FATTY ACIDS AND PIGMENTS CONTENT OF NANNOCHLOROPSIS OCULATA (EUSTIGMATOPHYCEAE) CULTURE AT BAG SYSTEMS USING DIFFERENT NITROGEN SOURCES AND CONCENTRATION IN MEDIUM
摘要：Effects of nitrogen sources (N〇3--N and NH4+-N and urea-N) and different concentrations (882, 441, 1323 and 1764 ^mol/L-1) on growth and biochemical composition of Nannochloropsis oculata were tested. The algae grown in both 882 and 441 ^mol/L concentrations of N〇3--N in the medium showed maximum cell numbers of 51.76±3.10x106 cells/ml and 26.38±3.07x106 cells/ml, respectively. On the other hand, NO3- concentration in the medium was increased 1323 ^mol/L and 1764 ^mol/L, maximum cell numbers were significantly increased 93.51±3.62x106 cells/ml and 112.29±11.64x106 cells/ml, respectively. The highest EPA content and total w-3 of fatty acids of alga were 29.83±1.50% and 38.00±1.86 % of the dry weight when N〇3--N concentration was 882 ^mol/L, respectively. Total carotenoid content of 882 ^mol/L in the concentration of N represented the highest value in the N. oculata biomass. With N〇3--N concentration at 1764 ^mol/L, the highest value for chlorophyll a was 12.04 ± 0.92 pg/ml. As a result, nutrient limitation severely reduced cellular growth rate, on the other hand, cellular growth rate increased by more nitrogen concentration. Biochemical composition, particularly EPA values, changed in accordance with not only NO3- -N, NH4+-N and Urea-N concentrations but also harvest time.
