摘要：A research was made on glyoxal oxidase, manganese peroxidase and laccase produced under the impact of Heterobasidion Insulare (HIJ-2234) medium components. The change in the concentrations of copper could positively affect the secretion of lignin-degrading enzyme. H. Insulare extracellular fluid would enable the maximum production of lignin (161mU/ml glyoxal oxidase, 46.01 U/ml laccase and 241.6 mU/ml MnP), which demonstrates the maximum capabilities of decolorizing dyes Ponceau 2R, anthraquinone blue, malachite green and Ponceau 2R (a xylidine derivative) (at a velocity of 2.41, 1.53 and 3.0 mg ml-1 h-1, separately). Simultaneously, the decolonization rate of the lignin and the activities of the enzyme were analyzed through multivariate regression. The experimental results meet the linear acceleration mode of interaction. Response surface was obtained through comparison, which manifests that manganese peroxidase activity plays a main role in malachite green decolorization even though laccase is of greater importance in dimethyl aniline degradation.
