摘要：The honeybees are vital to the continuity of almost all living things on earth. Unfortunately, honeybees are under the threat of many pathogens as Paenibacillus larvae. These pathogens cause severe loss in the hives resulting in bee colony extinction, which is a cause for international concern because it results in massive deaths of commercial honeybee stocks. Due to the economic and ecological importance of honeybees, it is essential to develop effective, sustainable and environmentally friendly strategies for the control of bee diseases. Among these strategies, the use of environmentally friendly biological control preparations which will be developed from its own microbiota is very important. With the idea that honeybees may be more resistant to diseases if the microbiota are supported, this study aimed to first determine the bacteria found in healthy honeybees and their products. In line with this goal, nineteen bacteria were identified with molecular techniques. These included: in the honey, Staphylococcus warneri (HD5, HD20); in the pollen, Lactobacillus kunkeei (HD6), Fructobacillus fructosus (HD8), S. lentus (HD9), Pantoea vagans (HD10), Bacillus licheniformis (HS6), Pluralibacter pyrinus (HS10) and P. anthophila (HS11); in bee bread, L. kunkeei (HD12), P. agglomerans (HD11), B. cereus (HS1 and HS3) and B. safensis (HS2 and HS4); and in the bees, B. safensis (HD18), Escherichia coli (HD13) and Enterobacter cloacae (HS19, HS20). Furthermore, it was determined that L. kunkeii (HD6 and HD12) and F. fructosus (HD8) had high inhibition activity against the American foulbrood agent P. larvae.
