摘要：Silva LA, Scheffer DL, Alves A, Pereira LT, Moneretto DB, Tromm C, Streck EI, Silveira PCL, Pinho RA. Effect of Aerobic Training of Moderate and Low Volume on Electron Transport Chain Activity and Oxidative Stress Markers in Skeletal Muscle. JEPonline 2015;18(6):81-93. The purpose of this study was to compare the adaptations induced by moderate and low volume aerobic exercise programs on mitochondrial enzyme activity oxidative stress markers in skeletal muscle of mice. Eighteen male mice (CF1) weighing 30 to 35 g were randomly distributed into three groups (n = 6): (a) untrained (UT); (b) moderate training volume (MTV); and (c) low training volume (LTV). Animals were submitted to an 8-wk training program. Forty-eight hours after the last training session the animals were killed by decapitation and the quadriceps muscles were removed and stored at –70º C. Succinate dehydrogenase (SDH), mitochondrial respiratory chain enzyme activities (complex I-II), thiobarbituric acid reactive species (TBARS), protein carbonyls (PC), superoxide dismutase (SOD), and catalase (CAT) were measured. Results indicate that MTV program increase SDH (19%) activity, complex I (56%) and II (67%) more than the LTV, Journal of Exercise Physiologyonline Editor-in-Chief Tommy Boone, PhD, MBA Review Board Todd Astorino, PhD Julien Baker, PhD Steve Brock, PhD Lance Dalleck, PhD Eric Goulet, PhD Robert Gotshall, PhD Alexander Hutchison, PhD M. Knight-Maloney, PhD Len Kravitz, PhD James Laskin, PhD Yit Aun Lim, PhD Lonnie Lowery, PhD Derek Marks, PhD Cristine Mermier, PhD Robert Robergs, PhD Chantal Vella, PhD Dale Wagner, PhD Frank Wyatt, PhD Ben Zhou, PhD Official Research Journal of the American Society of Exercise Physiologists ISSN 1097-9751 Official Research Journal of the American Society of Exercise Physiologists ISSN 1097-9751 82 when compared to untrained mice. However, the LTV program decreased oxidative damage (TBARS by 32% and CP by 22%) more than MTV, against UT. Antioxidant enzyme activity (SOD 71% / CAT 73%) increased similarly in both programs. In conclusion, MTV caused greater increases in mitochondrial respiratory chain enzyme activity as compared to LTV, while LTV caused a sharper decrease in oxidative stress in comparison to MTV.
