摘要：Hutchison AT, Stephanie, DG, Cassandra H. Norepinephrine Modulates Dendritic Cell Activity by Altering Chemokine Release. JEPonline 2010;13(1):33-40. Dendritic cells (DC) are antigen presenting cells of the immune system that mature in peripheral tissues after exposure to microbial antigens, such as lipopolysaccharide (LPS). Once activated, DC travel to the lymph nodes where they recruit and reciprocally activate other leukocytes by releasing chemokines. Specifically, MIP-1α, MIP-1β, and RANTES recruit natural killer (NK) cells via binding with the chemokine receptor CCR5. Physiological stress can increase the circulating concentration of NK cells. It has been speculated that the release of norepinephrine (NE), a “stress hormone” into the lymph nodes inhibits the leukocyte recruitment capacity of DC, thus allowing the NK cells to move into the circulation. Although previous observations have shown that NE can inhibit aspects of DC function, its effect on the production of MIP-1α, MIP-1β, and RANTES has not been determined. The purpose of this study was to assess the effect of NE on DC production of MIP-1α, MIP-1β, and RANTES. DC were incubated with LPS alone or with NE for 24-h. The supernatant concentration of each chemokine was assessed by ELISA. NE significantly inhibited the production of MIP-1β (-74%, P = 0.001) and RANTES (-93%, P < 0.001), but not MIP-1α (-17%, P = 0.227). These results support the contention that NE-mediated inhibition of DC function may play a role in leukocyte release from the lymph nodes into the circulation during physiological stress.
