摘要：Park ST, Kim K, Yoon JH, Lee S. Effect of Exercise on GLUT4 Expression of Skeletal Muscle in Streptozotocin-Induced Diabetic Rats. JEPonline 2011;14(4):113-122. The purpose of this study was to use the immunofluorescence method to identify whether exercise training increases GLUT4 expression in the cell membrane of skeletal muscle of streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley rats were randomly divided into four groups: control (CON); exercise (EX); diabetes mellitus (DM); and diabetes mellitus with exercise (DM+EX). The treadmill was used for exercise with moderate intensity for 30 min a day, 5 days a week for 6 weeks. We observed that subcellular distribution of GLUT4 determined by immunofluorescence microscopy is entirely located in the cell membrane of muscle fibers. In the DM group, minimal staining can be seen at the cell membrane, but the CON and EX groups show much more staining at the cell membrane. The DM+EX group showed more staining than the DM group. In addition, the results showed that the expression of the number of nuclei per muscle fiber was significantly decreased in the DM group (13.12±0.89) than in the CON group (19.37±0.56) (P<0.05). Also, exercise alleviated diabetes mellitus-induced decrease of nuclei per muscle fiber to the control level (19.56 ±1.07) (P<0.05). We identified that exercise training increased the expression of GLUT4 in the cell membrane and myonuclei in skeletal muscle fibers of STZinduced diabetic rats.
