摘要：Chemically activated luciferase expression (CALUX) is a reliable, fast and inexpensive bioassay technology for screening and analysing PCDD/Fs semi-quantitatively in various media. However, heavy metal ions (HMIs) may influence the luciferase expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) and cell activity in H1L6.1 cell lines, resulting in deviating measurement results. This study applied five different HMIs (Pb2+, Hg2+, Cd2+, Cu2+ and Zn2+) in a 2-level factorial design for exploring their impact on CALUX results. The results revealed that all five ions somewhat influenced luciferase expression and cell activity, yet Hg2+ had the greatest adverse impact on luciferase activity. Combined action of Hg2+ and Cu2+ prompted the most obviously negative effects on cell activity. The maximum tolerable level of the two critical metals for H1L6.1 cell lines was also determined (i.e., 1 ^g-Hg2+ or 2.5 ^g-Cu2+/ml-medium) by comparing the impact under continuous heavy metal concentration. The observed data revealed that the influence mechanism of heavy metals on luciferase activity is complicated, which is not only caused by the reduction of H1L6.1 cells, but also may be the inhibition effect of a metal on CYP1A1 activation and reduction of luciferase enzyme production.
关键词：H1L6.1 cell lines;Relative luciferase unit (RLU);Relative survival ratio (RSR);PCDD/Fs;CALUX bioassay;Cadmium;Copper;Lead;Mercury;Zinc
