摘要：Although the effect of nicotine in tissue damage has been demonstrated in many studies; little is known about the protective effects of vitamin-C, vitamin-E and beta-carotene treatment in the tissue damage that is induced by chronic nicotine exposure. Several studies demonstrate that vitamin C, vitamin E, and beta-carotene treatment prevents tissue damage in various models of inflammation. The aim of this study was to investigate the effects of vitamin-C, vitamin-E, and beta-carotene treatment on nicotine-induced oxidative changes in urinary system in rat, and to explore the possible mechanisms of action. Male Wistar albino rats were injected with nicotine 1.25 mg/kg i.p.) or saline for 28 days. Vitamin-C (125mg/kg b.wt. p.o.), vitamin-E (50mg/kg b.wt. p.o.), and/ or beta-carotene (40mg/kg b.wt. p.o.) were administered on alternative days. At the end of the treatment period kidney and bladder tissue samples were collected and examined histologically. Vitamin C, vitamin E, and beta-carotene supplementation to nicotine-treated animals, reversed partially or completely the cytotoxicity of nicotine in both urinary bladder and kidney tissues. These data suggest that various vitamins (antioxidant) supplementation effectively counteracts the deleterious effect of chronic nicotine administration in urinary system and attenuates oxidative damage possibly by its antioxidant effects.
关键词：Nicotine;Vitamins;Oxidative stress;Urinary system
