摘要：Preparation of immobilized enzyme carriers and their applications are a favourite branch of nanotechnology nowadays. Chitosan nanoparticles thin films (CSNPs-TFs) are applied as suitable supports for acid phosphatase immobilization to estimate heavy metals in water studied with UV-Vis spectrophotometric method, based on their inhibiting enzyme activity. According to the results, immobilization of acid phosphatase onto chitosan nanoparticles thin films, as compared with chitosan macro- and micro-particles, has improved the activity and performance of the enzyme. Under optimal conditions, the detection limits of biosensor towards Pb(II) and Cr(II) were respectively 2.75 and 2.03 mg/L. The reproducibility of the proposed biosensor was six-fold. The biosensor response was stable for at least three weeks. Compared to the commercial enzyme, the fresh natural biosensor presented better performance in metal evaluation. The biosensor-based acid phosphatase, immobilized on CSNPs-TFs, evaluated heavy metals with a rapid, cheap and easy method, compared with other reported heavy metal assays, and fresh natural enzyme had better activity towards the detection of heavy metal in water.
关键词：Acid Phosphatase;Biosensor;Chitosan nanoparticles;Heavy metals;Immobilization;Thin film
