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  • 标题:Isolation and Characterization of Three and Four Ring Pahs Degrading Bacteria from Contaminated Sites, Ankleshwar, Gujarat, India
  • 本地全文:下载
  • 作者:Jignasha G. Patel ; J.I. Nirmal Kumar ; Rita N Kumar
  • 期刊名称:International Journal of Environment
  • 印刷版ISSN:2091-2854
  • 出版年度:2015
  • 卷号:4
  • 期号:1
  • 页码:130-140
  • DOI:10.3126/ije.v4i1.12184
  • 语种:English
  • 出版社:Progressive Sustainable Developers Nepal (PSD-Nepal)
  • 摘要:Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from prolong contaminated Amalakhadi sediment and crude oil polluted soil Telva, near Ankleshwar Gujarat India. Organisms were treated with two-model PAHs compound Anthracene (ANT), and Pyrene (PYR) as the sole source of carbon and energy. Identification of the isolates was carried out based on their morphological and partial 16S rRNA gene sequences, which revealed that the isolates belong to two main bacterial groups: gram-negative pseudomonas indoxyladons and gram-positive, spore-forming group, Bacillus benzoevorans. GC-MS based degradation study demonstrated that P. indoxyladons efficiently degrade 98% of ANT and PYR by 93.2 % when treated with 250 mg L-1. However, B. benzoevorans could tolerate to 200 mg L-1of PYR. Thus, the findings of the study provide novel bacterial sp. having different capacity to degrade model PAHs compounds and further could be utilized for the standardization of bioremediation protocols for ex situ and in situ studies in aquatic as well as terrestrial ecosystem. DOI: http://dx.doi.org/10.3126/ije.v4i1.12184 International Journal of Environment Volume-4, Issue-1, Dec-Feb 2014/15, Page: 130-140
  • 其他摘要:Polycyclic aromatic hydrocarbon (PAH)-degrading bacteria were isolated from prolong contaminated Amalakhadi sediment and crude oil polluted soil Telva, near Ankleshwar Gujarat India. Organisms were treated with two-model PAHs compound Anthracene (ANT), and Pyrene (PYR) as the sole source of carbon and energy. Identification of the isolates was carried out based on their morphological and partial 16S rRNA gene sequences, which revealed that the isolates belong to two main bacterial groups: gram-negative pseudomonas indoxyladons and gram-positive, spore-forming group, Bacillus benzoevorans. GC-MS based degradation study demonstrated that P. indoxyladons efficiently degrade 98% of ANT and PYR by 93.2 % when treated with 250 mg L-1. However, B. benzoevorans could tolerate to 200 mg L-1of PYR. Thus, the findings of the study provide novel bacterial sp. having different capacity to degrade model PAHs compounds and further could be utilized for the standardization of bioremediation protocols for ex situ and in situ studies in aquatic as well as terrestrial ecosystem. DOI: http://dx.doi.org/10.3126/ije.v4i1.12184 International Journal of Environment Volume-4, Issue-1, Dec-Feb 2014/15, Page: 130-140  
  • 关键词:PAHs contaminated soil;Isolated Bacteria;Biodegradation of PAHs
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