摘要:Graphical abstractDisplay OmittedHighlights•A low-cost circuit to control up to 70 Solenoid valves.•A python package that allows users to control the circuit board with python programs.•A comprehensive GUI to write and share automated protocols for experiments: no programming experience is required.AbstractMicrofluidic devices with integrated valves provide precise, programmable fluid handling platforms for high-throughput biological or chemical assays. However, setting up the infrastructure to control such platforms often requires specific engineering expertise or expensive commercial solutions. To address these obstacles, we present a Kit for Arduino-based Transistor Array Actuation (KATARA), an open-source and low-cost Arduino-based controller that can drive 70 solenoid valves to pneumatically actuate integrated microfluidic valves. We include a python package with a GUI to control the KATARA from a personal computer. No programming experience is required.