期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2018
卷号：7
期号：1
页码：92-97
DOI：10.1016/j.bjbas.2017.07.010
语种：English
出版社：Elsevier
摘要：AbstractMethicillin-resistantStaphylococcus aureusis a problem in both healthcare institutions and community settings. This is due to its multi-drug resistant challenges. Hence, this study assessed the prevalence of methicillin resistant gene (mecA), exfoliative toxin (etaandetb) and toxic shock syndrome (tsst-1) genes inS.aureusisolated from clinical samples. A total of 120 clinical samples of patients (urine, high vagina swab (HVS), semen, wound swab, sputum and urethral swab) from a hospital laboratory were obtained.S.aureuswas isolated and then identified with API-staph kit. Antibiotic susceptibility of the isolates was determined by agar diffusion while PCR was used to detect the presence ofmecAand toxin-associated genes. FiftyS.aureusisolates were obtained at frequencies of 26(52%), 12(24%), 4(8%), 3(6%), 3(6%) and 2(4%) from the HVS, urine, semen, wound, sputum and urethral swab samples respectively. All the isolates ofS.aureuswere resistant to the antibiotics used in this study.MecA,tsst-1,etaandetbwere detected in 19(38%), 7(14%), 3(6%) and 2(4%) of the isolates respectively. The prevalence of MRSA and its resistance pattern observed in this study was a signal that the health-care workers and the general public are at risk.
