摘要：Park ST, Kim K, Yoon JH, Lee S. Effect of Exercise on GLUT4Expression of Skeletal Muscle in Streptozotocin-Induced DiabeticRats. JEPonline 2011;14(4):113-122. The purpose of this study wasto use the immunofluorescence method to identify whether exercisetraining increases GLUT4 expression in the cell membrane of skeletalmuscle of streptozotocin (STZ)-induced diabetic rats. Sprague-Dawleyrats were randomly divided into four groups: control (CON); exercise(EX); diabetes mellitus (DM); and diabetes mellitus with exercise(DM+EX). The treadmill was used for exercise with moderate intensityfor 30 min a day, 5 days a week for 6 weeks. We observed thatsubcellular distribution of GLUT4 determined by immunofluorescencemicroscopy is entirely located in the cell membrane of muscle fibers.In the DM group, minimal staining can be seen at the cell membrane,but the CON and EX groups show much more staining at the cellmembrane. The DM+EX group showed more staining than the DMgroup. In addition, the results showed that the expression of thenumber of nuclei per muscle fiber was significantly decreased in theDM group (13.12±0.89) than in the CON group (19.37±0.56) (P<0.05).Also, exercise alleviated diabetes mellitus-induced decrease of nucleiper muscle fiber to the control level (19.56 ±1.07) (P<0.05). Weidentified that exercise training increased the expression of GLUT4 inthe cell membrane and myonuclei in skeletal muscle fibers of STZinduceddiabetic rats.
