摘要:AbstractIntroductionMicrobiology is one of the basic sciences subject in public health education. Given the importance of this subject in the medical profession, this study performed to determine medical students and general practitioners (GPs) prospective on microbiology course application in Birjand University of Medical Sciences (BUMS). In this descriptive analytical study, all physiopathology students and interns in BUMS and all general practitioners with 2-10 years of experience were participated. A questionnaire containing questions about various applications of microbiology course, the best teaching procedures, the best time for teaching section and the importance of teaching each lesson were completed and were analyzed using chi-square test at significance level α=0.05. In the present study, 162 persons (27 GPs, 71 interns and 64 physiopathology students) participated. The majority of physicians (70.4%) believed that general microbiology exerts a low impact in clinical judgment and professional practice, while 81.5% GPs and 56.3% of the interns believed that systematic part of microbiology reveals high influence in this regards. Almost all the participants (95.5%) believed that time allocated to the microbiology teaching is suitable. The majority (52.5%) of them thought that basic sciences section and clinical section are the best time for teaching the general and systematic parts of microbiology, respectively. More than forty percents of them (41.4%) believed that group discussion is the best procedure for teaching. Microbiology course is very useful in medical practice. It is recommended to emphasis more on clinical relevance and applicable topics at higher sections. Moreover, modern procedures and new strategies should be presented in the teaching process.