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  • 标题:Glycan-specific whole cell affinity chromatography: A versatile microbial adhesion platform
  • 本地全文:下载
  • 作者:Maxwell L. Van Tassell ; Neil P.J. Price ; Michael J. Miller
  • 期刊名称:MethodsX
  • 印刷版ISSN:2215-0161
  • 电子版ISSN:2215-0161
  • 出版年度:2014
  • 卷号:1
  • 页码:244-250
  • DOI:10.1016/j.mex.2014.10.005
  • 语种:English
  • 出版社:Elsevier
  • 摘要:Graphical abstractAbstractWe have sought a universal platform for elucidating and exploiting specificity of glycan-mediated adhesion by potentially uncharacterized microorganisms. Several techniques exist to explore microbial interactions with carbohydrate structures. Many are unsuitable for investigating specific mechanisms or uncharacterized organisms, requiring pure cultures, labeling techniques, expensive equipment, or other limitations such as questionable stability, stereospecificity, or scalability. We have adapted an affinity chromatography resin as a model to overcome these drawbacks, among others. It readily allows for the quantification, selection, and manipulation of target organisms based on interactions with glycan ligands. To maximize its utility as a selective screening method, we have constructed the tool such that it:•Promotes whole-cell interactions using viable, unaltered cells.•Provides robust spatial interactions with target glycans, presented with controlled stereo-specificity, for high affinity/avidity interactions that reflect a complexin vivomatrix.•Has the ability to utilize any reducing glycan, is quick, efficient, safe, and affordable to construct, and is scalable and reusable for multiple applications.
  • 关键词:Affinity chromatography;Bacterial adhesion;Carbohydrate modification;Screening;Lectins;Escherichia coli;Campylobacter jejuni
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