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  • 标题:A resource from 3D electron microscopy of hippocampal neuropil for user training and tool development
  • 本地全文:下载
  • 作者:Kristen M. Harris ; Josef Spacek ; Maria Elizabeth Bell
  • 期刊名称:Scientific Data
  • 电子版ISSN:2052-4463
  • 出版年度:2015
  • 卷号:2
  • DOI:10.1038/sdata.2015.46
  • 语种:English
  • 出版社:Nature Publishing Group
  • 摘要:Resurgent interest in synaptic circuitry and plasticity has emphasized the importance of 3D reconstruction from serial section electron microscopy (3DEM). Three volumes of hippocampal CA1 neuropil from adult rat were imaged at X-Y resolution of ~2 nm on serial sections of ~50–60 nm thickness. These are the first densely reconstructed hippocampal volumes. All axons, dendrites, glia, and synapses were reconstructed in a cube (~10 μm3) surrounding a large dendritic spine, a cylinder (~43 μm3) surrounding an oblique dendritic segment (3.4 μm long), and a parallelepiped (~178 μm3) surrounding an apical dendritic segment (4.9 μm long). The data provide standards for identifying ultrastructural objects in 3DEM, realistic reconstructions for modeling biophysical properties of synaptic transmission, and a test bed for enhancing reconstruction tools. Representative synapses are quantified from varying section planes, and microtubules, polyribosomes, smooth endoplasmic reticulum, and endosomes are identified and reconstructed in a subset of dendrites. The original images, traces, and Reconstruct software and files are freely available and visualized at the Open Connectome Project (Data Citation 1: Open Connectome Project http://dx.doi.org/10.7281/T11Z429Q).
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