摘要：AbstractTo further test the accuracy and rationality of the Chinese classification system for chrysanthemum (Chrysanthemum morifolium), 10 simple sequence repeat (SSR) markers were used to identify a collection of 88 chrysanthemum and its related genera accessions. In total, 42 effective alleles across 88 accessions were detected; 3 429 bands were obtained by PCR amplification, including 2 630 polymorphic bands. The similarity coefficient ranged from 0.53 to 0.88. Cluster analysis based on UPGMA illustrated that the wild species and large-flower cultivars were first divided into two clusters, then the large-flower cultivars formed five distinct groups according to petal type, indicated that petal type can be a classification criterion. In the wild species cluster,C. vestitumandC. zawadaskiigrouped withA. trilobata, suggested that theAjaniagenera was closely related to theChrysanthemumgenera. ‘Hangbaiju’, ‘Gongju’ and ‘Chuju’ were grouped together, and ‘Boju’,O. longilobusandC. mongolicumconstituted another branch, showed a correlation with geographic region of origin. Population structure analysis was subsequently performed withKvalues ranging from 2 to 10, and the most likely estimate for the population structure is five subpopulations, which is nearly consistent with the clustering results. Principle component analysis was further performed to verify the classification results. The results showed that these SSR markers are very powerful for studying genetic relationships and will be useful tools in the identification and classification of chrysanthemum.
