摘要：AbstractBioactive flavonoids derived fromAsplenium nidus(fern) possess potent tumoricidal, anti-bacterial, anti-oxidant activities that are responsible for their chemo-preventive potential against multidrug-resistant (MDR) pathogens,Proteus mirabilis, Proteus vulgarisandPseudomonas aeruginosa. Fractionation and recognition of the flavonoids were attained through gas chromatography and mass spectrometry (GC/MS). Twelve known and three unknown compounds were revealed by fractions 1 & 3, representing 13.12% and 2.61% out of the total composition (15.12%). Gliricidin7-O-hexoside was found 3.83% followed by quercetin-7-O-rutinoside (3.09%) keampferol-3-O-rutinoside (0.19%) and myricetin-3-O-rhamnoside (1.10%). The anti-bacterial and anti-oxidant activities of present fractions along with pure bioactive constituents were tested against three MDR pathogens through microbroth dilution: MIC50ranges for amoxicillin, gliricidin7-O-hexoside and quercetin 7-O-rutinoside were 0.0003–0.06μg/mL, 0.004–0.06μg/mL and 0.005–6.0μg/mL, respectively. Gliricidin-7-O-hexoside and quercetin-7-O-rutinoside’s inhibitory activities were comparable to standard antibiotic, amoxicillin (p>0.05). Herein we report for the first time a number of flavonoids that may act as the source of therapeutically useful compounds against MDR pathogens. In addition, the most extracted flavonoids (gliricidin-7-O-hexoside (78.1%) and quercetin-7-O-rutinoside (69.2%) showed a significantin vitroantioxidant activity i.e. DPPH radical scavenging activity. Both fractions ofA. nidusshowed obvious cytotoxic effects on human hepatoma HepG2 and human carcinoma HeLa cells. Moreover, the anti-cancer activity generally enhanced with ameliorating antioxidant and antibacterial potential of fern’s flavonoids. These findings illustrate the potential of this fern as a probable source of bioactive constituents and provide a scientific basis for its folklore or ethno-medicinal uses for infectious diseases and cancers.
