标题：Antimicrobial sensitivity, extended spectrum beta-lactamase (ESBL) production and plasmid profile by microorganisms from Otitis media patients in Owo and Akure, Ondo State, Nigeria
期刊名称：Karbala International Journal of Modern Science
印刷版ISSN：2405-609X
电子版ISSN：2405-609X
出版年度：2018
卷号：4
期号：3
页码：332-340
DOI：10.1016/j.kijoms.2018.07.001
语种：English
出版社：Elsevier
摘要：AbstractMicrobiological analyses were carried out on clinical samples from Otitis media patients attending two government hospitals in Akure and Owo, Ondo State, Nigeria. Bacteria isolates such asEscherichia coli,Klebsiella pneumoniae,Proteus mirabilis,Pseudomonas aeruginosa,Staphylococcus aureus,Streptococcus pneumoniae,Staphylococcus epidermidis,Streptococcus pyogenesand fungal isolates such asAspergillus niger,Aspergillus fumigatus,Aspergillus flavusandCandida albicanswere identified from the analyzed samples. All the bacteria isolates were screened for beta lactamase production of which varying percentages of the characterized isolates were positive for beta-lactamase production such asE. coli(12.1%),K. pneumoniae(16.7%),P. mirabilis(8.2%),P. aeruginosa(28.8%),S. aureus(11.1%),Strep. pneumoniae(13.3%),S. epidermidis(7.1%) andStrep. pyogenes(10.7%), respectively. The results obtained from antibiotic sensitivity revealed the presence of multiple antibiotic resistant isolates (MDRIs) present among the bacteria isolates obtained and that Nitrofurantoin and Ofloxacin were the most effective antibiotics against the bacteria isolates tested. The Multiple Drug Resistant Isolates were subjected to plasmid profile analysis and they were cured of their resistant factor plasmids; they were subjected to post-plasmid curing antibiotic resistant assay where it was discovered that their initial antibiotic resistance was plasmid-mediated. Furthermore, the antifungal sensitivity test of fungal isolates revealed that Fluconazole and Clotrimazole were the most effective antifungal agents against the fungal isolates obtained from the samples analyzed.
关键词：Otitis media;Antibiotic sensitivity patterns;Antifungal sensitivity;Plasmid profile analysis;Beta-lactamase production
