摘要：Graphical abstractDisplay OmittedAbstractThe present study is designed to identify various bioactive flavonoid compounds from the methanolic fern extract (MFE) ofCheilanthes tenuifolia. Flavonoids derived fromC. tenuifolia(fern) possess potent anti-cancerous, anti-bacterial, anti-oxidant activities that are responsible for their chemo-preventive potential against selected bacterial panel. A preparative column chromatography with a column Sephadex LH-20 was used to isolate and purify flavonoids fromC. tenuifolia.Their structure and chemical bonds were identified by using Nuclear Magnetic Resonance (NMR) spectroscopy and Fourier Transform-Infra Red Spectroscopy (FTIR). Two flavonoids were identified as rutin (2.8mg) and quercetin (3.34mg) from 100g ofC. tenuifolia. The minimum inhibitory concentration (MIC) values of (2.25 and 0.45µg/ml) were obtained in purified flavonoids againstStaphylococcus aureusandEnterobacter sp., respectively. The two flavonoids (Rutin and quercetin) showed significantin vitroanti-oxidant activity; in this regard, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging potential of quercetin (86.1%) was higher than that of rutin (73.2%). However, for human hepatoma HepG2 and human carcinoma HeLa cells, quercetin exhibited high anti-cancer activity than rutin. The purified compounds (rutin and quercetin) with having great potential of free radical inactivator in HepG2 cells. The results suggest that MFE ofC. tenuifoliacould potentially be employed in traditional medicine as they are rich in compounds with anti-oxidant, anti-microbial and anti-cancer properties.
关键词：Cheilanthes tenuifolia;Column chromatography;Rutin;Quercetin;Fourier Transform-Infra Red Spectroscopy;Nuclear Magnetic Resonance
