摘要：AbstractMethanolic extract of flowers ofVinca major(VFE) reduced the activity of porcine pancreatic lipase (PPL) which was reported in our previous study. To identify the antilipase molecule(s) in the VFE, the antilipase activity-guided fractionation for the purification of lipase inhibitory molecule(s) by Silica Gel and Sephadex G-50 column chromatography was attempted, and the final anti-lipase fraction was examined by ESI-MS and FT-IR spectroscopy. Two molecules namely, Majoridine and Akuammine were elucidated to be present in the flowers ofV. majorwhich possessed high PPL inhibition activity. Further, both molecules were lookedin silicofor their interactions with PPL and other important proteins of lipid metabolism using molecular docking studies. Majoridine and Akuammine demonstrated significant binding interactions with active site residues of PPL.
