摘要:Plasma-activated medium (PAM) has been produced by exposing a liquid media to Argon plasma jet. The jet plasma exposure to liquid media has produced reactive Oxygen species (ROS) in liquid phase. This study aims to determine the number of reactive species in plasma-activated medium. An atmospheric pressure plasma jet (APPJ) was generated with a dielectric barrier discharge (DBD) column by AC high voltage. Some parameters varied including exposure time; i.e. 5, 10, 15, 20, 25, and 30 min; and the distance between reactor and active media; i.e. 1, 2 and 3 cm. Some analysis conducted including variation of exposure times, the distances of reactor to PAM which affect produced concentration, and the reactive species composition in plasma-activated medium. In addition, temperature characteristics, pH levels, dissolved ozone and dissolved hydrogen peroxide concentrations were also observed in this study. The results showed that increased exposure time resulted in decreased pH, increased temperature and increased concentrations of ozone and hydrogen peroxide. The maximum reactive species composition was obtained at the distance between reactor and plasma-activated medium of 2 cm. Maximum reactive species composition obtained in this study has temperature of 29-30 Celsius degrees; pH 3.5; dissolved ozone 2.97 ppm; and Hydrogen Peroxide 215 ppm.