期刊名称：Beni-Suef University Journal of Basic and Applied Sciences
印刷版ISSN：2314-8535
电子版ISSN：2314-8543
出版年度：2013
卷号：2
期号：2
页码：103-107
DOI：10.1016/j.bjbas.2013.03.001
语种：English
出版社：Elsevier
摘要：AbstractMycoplasma bovisis a major bacterial pathogen causing widespread respiratory disease, mastitis, and arthritis in cattle. Ineffective drugs treatments, culling of infected cows and loss of milk production can lead to significant economic losses in dairy farms. The early detection of cows excretingM. bovisbacteria to prevent mastitis outbreak is necessary. In our study the percentage ofM. bovisinfection among dairy herd was recorded by cultivation in combination with digitonin test and confirmed by species specific PCR as 4%. The protein profile of the isolated field German strains was compared toM. bovisreference strain Donetta PG45 using SDS-PAGE. The pattern of the reference strain was different from that of field strains as well as a marked heterogenicity among the field strains was noticed.
关键词：Mycoplasma bovis;SDS-PAGE;Species specific PCR
