摘要：Graphical abstractDisplay OmittedAbstractA multiplex PCR was described to simultaneously detectmcr-1and frequently occurring carbapenem-resistant genes includingblaKPC, blaNDM, blaIMP, andblaOXA-48-likein a single reaction. The PCR product sizes of these 4 carbapenem-resistant genes were 232 bp, 438 bp, 621 bp, and 798 bp forblaIMP,blaOXA-48-like,blaNDM, andblaKPC, respectively, whereasmcr-1revealed 1126 bp of PCR product. This protocol accurately detected those resistant genes in agreement with the reference strains, 127 local carbapenem-resistant Enterobacteriaceae, 8mcr-1carrying Enterobacteriaceae, and 62 carbapenem-susceptible Enterobacteriaceae. This method will be useful for laboratory application and surveillance of carbapenem and/or colistin-resistant bacteria.
